منابع مشابه
Positional sequencing by hybridization
Sequencing by hybridization (SBH) is a promising alternative to the classical DNA sequencing approaches. However, the resolving power of SBH is rather low: with 64kb sequencing chips, unknown DNA fragments only as long as 200 bp can be reconstructed in a single SBH experiment. To improve the resolving power of SBH, positional SBH (PSBH) has recently been suggested; this allows (with additional ...
متن کاملLarge Scale Identification of SSR Molecular Markers in Ajowan (Trachyspermum ammi) Using RNA Sequencing
The medicinal plant, Trachyspermum ammi is a rich source of active pharmaceutical ingredients with pharmaceutics effects. Microsatellite markers play a key role in the genome and gene expression, especially in secondary metabolite biosynthesis in medicinal plants. For the first time, transcriptome sequencing of this herb medicine was carried out to identify the microsatellite markers of this sp...
متن کاملFungal community analysis by large-scale sequencing of environmental samples.
Fungi are an important and diverse component of soil communities, but these communities have proven difficult to study in conventional biotic surveys. We evaluated soil fungal diversity at two sites in a temperate forest using direct isolation of small-subunit and internal transcribed spacer (ITS) rRNA genes by PCR and high-throughput sequencing of cloned fragments. We identified 412 sequence t...
متن کاملSequencing by hybridization in the presence of hybridization errors.
DNA sequencing is a very important problem in genomics. Several different sequencing methods are currently utilized. One promising method uses a sequencing chip to obtain information about the presence of subsequences in DNA. This paper deals with sequencing of hybridization data from a sequencing chip, called Sequencing by Hybridization (SBH). Preparata et al. proposed a new sequencing chip us...
متن کاملAdaptive Control of Hybridization Noise in Dna Sequencing-by-hybridization
We consider the problem of sequence reconstruction in sequencing-by-hybridization in the presence of spectrum errors. As suggested by intuition, and reported in the literature, false-negatives (i.e., missing spectrum probes) are by far the leading cause of reconstruction failures. In a recent paper we have described an algorithm, called "threshold-theta", designed to recover from false negative...
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ژورنال
عنوان ژورنال: Journal of Computational Biology
سال: 2002
ISSN: 1066-5277,1557-8666
DOI: 10.1089/10665270252935548